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POCKET PCR INTEGRATES MICROFLUIDICS, ISOTHERMAL AMPLIFICATION, AND NUCLEIC ACID CHROMATOGRAPHY TECHNOLOGIES FOR EFFICIENT AND SENSITIVE DETECTION OF SPECIFIC VIRAL RNA FRAGMENTS. WITH A USER-FRIENDLY DESIGN, IT DELIVERS RESULTS WITHIN 30 MINUTES, MAKING IT IDEAL FOR RAPID DETECTION SCENARIOS.
REAL-TIME QUANTITATIVE THERMAL CYCLER (QPCR) IS A MOLECULAR TECHNIQUE THAT ENABLES THE DETECTION AND QUANTIFICATION OF SPECIFIC DNA SEQUENCES, OFFERING HIGH SENSITIVITY, ACCURACY, AND A DYNAMIC RANGE THAT MAKES IT A STANDARD IN MOLECULAR DIAGNOSTICS AND GENE EXPRESSION ANALYSIS. IT OPERATES BY MONITORING THE FLUORESCENCE SIGNAL IN REAL TIME AS THE PCR AMPLIFICATION PROGRESSES, ALLOWING FOR THE QUANTIFICATION OF THE TARGET DNA BASED ON THE CYCLE THRESHOLD (CT) VALUES. THIS METHOD IS WIDELY APPLIED ACROSS VARIOUS FIELDS, INCLUDING MEDICAL DIAGNOSTICS, RESEARCH, AND FORENSICS.